Plant Transcription Factor Database
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT3G15030.1
Common NameK15M2.17, MEE35, TCP4
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family TCP
Protein Properties Length: 420aa    MW: 45963.4 Da    PI: 7.2936
Description TCP family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT3G15030.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
          TCP   2 agkkdrhskihTkvggRdRRvRlsaecaarfFdLqdeLGfdkdsktieWLlqqakpaikeltgt........................ssssasecea 75 
                  +g+kdrhsk++T++g+RdRRvRlsa++a++f+d+qd+LGfd++sk+++WL+++ak++i+el+++                        +++s+s   +
                  79**************************************************************9999999999988888887766652222222233 PP

          TCP  76 esssssas.........nsssg........................kaaksaakskksqksaasalnlakesrakara 120
                  ++++++++             g                         ++ +   +++++++++  +nl+++ +++   
  AT3G15030.1 141 QQQQQQQQlqfgvgfngG---GaehpsnnessflppsmdsdsiadtIKSFFPVIGSSTEAPSN--HNLMHNYHHQHPP 213
                  333333333344444440...044444445667777777777776644444444444444444..5555555544444 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF036342.6E-3243147IPR005333Transcription factor, TCP
PROSITE profilePS5136934.01645103IPR017887Transcription factor TCP subgroup
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009793Biological Processembryo development ending in seed dormancy
GO:0009965Biological Processleaf morphogenesis
GO:0030154Biological Processcell differentiation
GO:0045962Biological Processpositive regulation of development, heterochronic
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000056anatomyflower bud
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 420 aa     Download sequence    Send to blast
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.237640.0floral meristem| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Expression AtlasAT3G15030-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in cotyledons during embryogenesis. Expressed during ovule development (PubMed:25378179). {ECO:0000269|PubMed:25378179}.
UniprotTISSUE SPECIFICITY: Expressed in cotyledons, particularly in the vascular region, in leaves, roots, buds, flowers and immature siliques. {ECO:0000269|PubMed:17307931}.
Functional Description ? help Back to Top
Source Description
TAIRArabidopsis thaliana TCP family transcription factor. Regulated by miR319. Involved in heterchronic regulation of leaf differentiation.
UniProtPlays a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes such as CUC genes, probably through the induction of miRNA (e.g. miR164). Required during early steps of embryogenesis. Participates in ovule develpment (PubMed:25378179). {ECO:0000269|PubMed:12931144, ECO:0000269|PubMed:15634699, ECO:0000269|PubMed:17307931, ECO:0000269|PubMed:25378179}.
Function -- GeneRIF ? help Back to Top
  1. a miRNA-resistant version of TCP4, when expressed in petals and stamens, causes these organs not to develop
    [PMID: 20007771]
  2. Arabidopsis plants with an increased activity of the transcription factor TCP4, which reduces cell proliferation in leaves, have higher miR396 and lower GRF levels.
    [PMID: 20023165]
  3. hyper-activation of TCP4 revealed its role in determining the timing of crucial developmental events, both at the organ and organism level.
    [PMID: 21518050]
  4. TCP4 protein blocks cell cycle of budding yeast, specifically at G1->S transition, by regulating G1 checkpoint control pathway.
    [PMID: 21658370]
  5. These analyses indicate a role for auxin, gibberellic acid and abscisic acid in TCP4-mediated control of leaf growth.
    [PMID: 21904111]
  6. Data show that the expression of TCP4 and IAMT1 that encodes a methyltransferase converting free indole-3-acetic acid (IAA) to methyl-IAA ester (MeIAA) was induced and the induction level was dependent on the abundance of TaWRKY71-1 transcripts.
    [PMID: 23671653]
  7. A small increase in TCP4 activity has an immediate impact on leaf cell number, by significantly reducing cell proliferation through miR319.
    [PMID: 25053833]
  8. Data showed that SPOROCYTELESS (SPL) most likely functions as a transcription repressor by interacting with TCP family transcription factors and the TOPLESS (TPL) co-repressors.
    [PMID: 25527103]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
Motif logo
Cis-element ? help Back to Top
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by the miRNA miR-JAW. {ECO:0000269|PubMed:12931144}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
Interaction ? help Back to Top
Source Intact With
BioGRIDAT3G02150, AT3G27010, AT3G47620, AT4G18390, AT4G27330, AT5G08070, AT5G08330, AT5G51910, AT5G60970, AT1G30210, AT1G35560, AT1G53230, AT1G58100, AT1G65620, AT1G69690
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT3G15030
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK3167040.0AK316704.1 Arabidopsis thaliana AT3G15030 mRNA, complete cds, clone: RAFL06-89-F15.
GenBankAP0003700.0AP000370.1 Arabidopsis thaliana genomic DNA, chromosome 3, TAC clone:K15M2.
GenBankAY0944440.0AY094444.1 Arabidopsis thaliana AT3g15030/K15M2_17 mRNA, complete cds.
GenBankAY1499570.0AY149957.1 Arabidopsis thaliana At3g15030/K15M2_17 mRNA, complete cds.
GenBankCP0026860.0CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001189896.10.0transcription factor TCP4
RefseqNP_850589.10.0transcription factor TCP4
RefseqNP_188121.10.0transcription factor TCP4
SwissprotQ8LPR50.0TCP4_ARATH; Transcription factor TCP4
TrEMBLB9DFB40.0B9DFB4_ARATH; AT3G15030 protein
STRINGAT3G15030.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
Representative plantOGRP18015163
Publications ? help Back to Top
  1. Cubas P,Lauter N,Doebley J,Coen E
    The TCP domain: a motif found in proteins regulating plant growth and development.
    Plant J., 1999. 18(2): p. 215-22
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
  3. Palatnik JF, et al.
    Control of leaf morphogenesis by microRNAs.
    Nature, 2003. 425(6955): p. 257-63
  4. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
  5. Souret FF,Kastenmayer JP,Green PJ
    AtXRN4 degrades mRNA in Arabidopsis and its substrates include selected miRNA targets.
    Mol. Cell, 2004. 15(2): p. 173-83
  6. Pagnussat GC, et al.
    Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis.
    Development, 2005. 132(3): p. 603-14
  7. Qin G, et al.
    An indole-3-acetic acid carboxyl methyltransferase regulates Arabidopsis leaf development.
    Plant Cell, 2005. 17(10): p. 2693-704
  8. Aguilar-Martínez JA,Poza-Carrión C,Cubas P
    Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds.
    Plant Cell, 2007. 19(2): p. 458-72
  9. Koyama T,Furutani M,Tasaka M,Ohme-Takagi M
    TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis.
    Plant Cell, 2007. 19(2): p. 473-84
  10. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
  11. Efroni I,Blum E,Goldshmidt A,Eshed Y
    A protracted and dynamic maturation schedule underlies Arabidopsis leaf development.
    Plant Cell, 2008. 20(9): p. 2293-306
  12. Nag A,King S,Jack T
    miR319a targeting of TCP4 is critical for petal growth and development in Arabidopsis.
    Proc. Natl. Acad. Sci. U.S.A., 2009. 106(52): p. 22534-9
  13. Rodriguez RE, et al.
    Control of cell proliferation in Arabidopsis thaliana by microRNA miR396.
    Development, 2010. 137(1): p. 103-12
  14. Aggarwal P, et al.
    Identification of specific DNA binding residues in the TCP family of transcription factors in Arabidopsis.
    Plant Cell, 2010. 22(4): p. 1174-89
  15. Koyama T,Mitsuda N,Seki M,Shinozaki K,Ohme-Takagi M
    TCP transcription factors regulate the activities of ASYMMETRIC LEAVES1 and miR164, as well as the auxin response, during differentiation of leaves in Arabidopsis.
    Plant Cell, 2010. 22(11): p. 3574-88
  16. Sarvepalli K,Nath U
    Hyper-activation of the TCP4 transcription factor in Arabidopsis thaliana accelerates multiple aspects of plant maturation.
    Plant J., 2011. 67(4): p. 595-607
  17. Aggarwal P, et al.
    The TCP4 transcription factor of Arabidopsis blocks cell division in yeast at G1→S transition.
    Biochem. Biophys. Res. Commun., 2011. 410(2): p. 276-81
  18. Sarvepalli K,Nath U
    Interaction of TCP4-mediated growth module with phytohormones.
    Plant Signal Behav, 2011. 6(10): p. 1440-3
  19. Causier B,Ashworth M,Guo W,Davies B
    The TOPLESS interactome: a framework for gene repression in Arabidopsis.
    Plant Physiol., 2012. 158(1): p. 423-38
  20. Viola IL,Reinheimer R,Ripoll R,Manassero NG,Gonzalez DH
    Determinants of the DNA binding specificity of class I and class II TCP transcription factors.
    J. Biol. Chem., 2012. 287(1): p. 347-56
  21. Li Z,Li B,Shen WH,Huang H,Dong A
    TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana.
    Plant J., 2012. 71(1): p. 99-107
  22. Yang X, et al.
    Evolution of double positive autoregulatory feedback loops in CYCLOIDEA2 clade genes is associated with the origin of floral zygomorphy.
    Plant Cell, 2012. 24(5): p. 1834-47
  23. Mecchia MA,Debernardi JM,Rodriguez RE,Schommer C,Palatnik JF
    MicroRNA miR396 and RDR6 synergistically regulate leaf development.
    Mech. Dev., 2013. 130(1): p. 2-13
  24. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
  25. Qin Z, et al.
    Ectopic expression of a wheat WRKY transcription factor gene TaWRKY71-1 results in hyponastic leaves in Arabidopsis thaliana.
    PLoS ONE, 2013. 8(5): p. e63033
  26. Danisman S, et al.
    Analysis of functional redundancies within the Arabidopsis TCP transcription factor family.
    J. Exp. Bot., 2013. 64(18): p. 5673-85
  27. Zhao J,Favero DS,Peng H,Neff MM
    Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by interacting with each other via the PPC/DUF296 domain.
    Proc. Natl. Acad. Sci. U.S.A., 2013. 110(48): p. E4688-97
  28. Schommer C,Debernardi JM,Bresso EG,Rodriguez RE,Palatnik JF
    Repression of cell proliferation by miR319-regulated TCP4.
    Mol Plant, 2014. 7(10): p. 1533-44
  29. Wei B, et al.
    The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis ovule development.
    Cell Res., 2015. 25(1): p. 121-34
  30. Chen GH,Sun JY,Liu M,Liu J,Yang WC
    SPOROCYTELESS is a novel embryophyte-specific transcription repressor that interacts with TPL and TCP proteins in Arabidopsis.
    J Genet Genomics, 2014. 41(12): p. 617-25